MiRNA-140-5p Modulates Cartilage Mechanical Environment by Preserving Surface Stress Homeostasis.

ABSTRACT: IntroductionThis study explores the interplay between miRNA-140-5p expression and mechanical stress in cartilage within joint biomechanics.MethodsTibial plateau specimens, CT data, and mechanical parameters were obtained from healthy and OA donors. Twenty-four mice, including 12 miRNA-140-5p knockdown (MUT-group) and 12 wild-type (WT group), underwent anterior-cruciate-ligament-transection (ACLT) or sham-operation (SHAM). Finite element analysis, 3D simulation, CT scans, immunohistochemical staining, and fluorescence in situ hybridization were conducted. Primary chondrocytes with or without miRNA-140-5p agomir were loaded mechanically and analyzed by RT-qPCR, Western blot, and phalloidin staining.ResultsThe mechanical coupling unit comprised articular cartilage and subchondral bone, with c

SUBMITTER: Li B 

PROVIDER: S-EPMC12678136 | biostudies-literature | 2025 Dec

REPOSITORIES: biostudies-literature