Comparative evaluation of two DNA methylation assays for triage of hrHPV E6/E7 mRNA-positive women.

ABSTRACT:

Background

The low specificity of high-risk human papillomavirus (hrHPV) testing necessitates effective triage strategies to avoid unnecessary colposcopy. DNA methylation testing shows promise, but its performance specifically in hrHPV E6/E7 mRNA-positive women, a population with active oncogenic activity, requires further evaluation.

Methods

This study evaluated the clinical performance of two commercial methylation-specific PCR assays, GynTect^® and CISCER^®, as triage tools for detecting high-grade cervical intraepithelial neoplasia (CIN) and cervical cancer in hrHPV E6/E7 mRNA-positive women. A total of 119 women were categorized into five groups based on colposcopy results: cervicitis, CIN1, CIN2, CIN3, and cervical cancer.

Results

Both GynTec

SUBMITTER: Yang Q 

PROVIDER: S-EPMC12678232 | biostudies-literature | 2025

REPOSITORIES: biostudies-literature