Transcriptome-wide m6A landscape across diverse porcine tissues revealed by nanopore direct RNA sequencing.

ABSTRACT: N6-methyladenosine (m6A) is the most prevalent internal RNA modification in eukaryotic messenger RNA (mRNA). Pigs are valuable not only as a source of meat protein but also as ideal animal models for studying human diseases. To date, m6A has not been systematically mapped in a body-wide survey of porcine tissues. In this study, we used direct RNA sequencing data of 39 sow samples (from 23 tissues) and 7 fetal samples (from 7 tissues) to identify m6A modifications and alternative splicing (AS) events. In total, we identified 60,823 transcripts, including 27,823 novel isoforms. The mean poly(A) tail length varied markedly among tissues, ranging from 48 to 101 nt. A total of 343,951 m6A sites were identified, with sow and fetal samples averaging 80,336 and 92,476 sites, respectively. The numb

SUBMITTER: Liao Y 

PROVIDER: S-EPMC12678253 | biostudies-literature | 2025

REPOSITORIES: biostudies-literature