InDAGO: a user-friendly interface for seamless dual and bulk RNA-Seq analysis.

ABSTRACT: Dual RNA-sequencing enables simultaneous profiling of protein-coding and non-coding transcripts from two interacting organisms, an essential capability when physical separation is difficult, such as in host-parasite or cross-kingdom interactions (e.g., plant-plant or host-pathogen systems). By allowing in silico separation of mixed reads, dual RNA-seq reveals the transcriptomic dynamics of both partners during interaction. However, existing analysis workflows often require programming expertise, limiting accessibility. We present inDAGO, a free, open-source, cross-platform graphical user interface designed for biologists without coding skills. inDAGO supports both bulk and dual RNA sequencing, with dual RNA sequencing further accommodating both sequential and combined approaches. Th

SUBMITTER: Aufiero G 

PROVIDER: S-EPMC12678335 | biostudies-literature | 2025

REPOSITORIES: biostudies-literature