Inducible chromosomal rearrangement reveals nonlinear polygenic dosage effects in driving aneuploid yeast traits.

ABSTRACT: Aneuploidy induces chromosomal scale alterations in gene dosage, impacting organismal proliferation yet serving as a driver for adaptive evolution. The complexity of gene dosage effects makes it challenging to elucidate the causal genetic basis of aneuploid consequence. Here, using loss-of-function screening with Synthetic Chromosome Rearrangement and Modification by LoxP-mediated Evolution (SCRaMbLE) in synthetic aneuploid yeast, in conjunction with gain-of-function testing in euploid yeast, we established a sufficient and necessary framework and discovered cases of nonlinear polygenic dosage effects in driving aneuploid phenotypes. We identified an emergent effect resulting from copy number alterations in a locus of five genes, which enhance trehalose biosynthesis and confer heat toleran

SUBMITTER: Yin H 

PROVIDER: S-EPMC12678449 | biostudies-literature | 2025 Dec

REPOSITORIES: biostudies-literature