Effect of antiserum against recombinant EGr_09888 on the in vitro development of Echinococcus granulosus protoscoleces.

ABSTRACT: To investigate the effects of Echinococcus granulosus sensu stricto (E. granulosus s.s.) reproduction-associated protein EGr_09888 on the development of E. granulosus s.s. protoscoleces (PSCs) in vitro. SWISS-MODEL, Netphos3.1, and PyMOL bioinformatics analysis software, along with subcellular localization prediction, were used to analyze the structure, phosphorylation sites, epitopes, and distribution of the EGr_09888 protein. The recombinant plasmid pET30a-EGr_09888 was constructed for protein expression and purification, and New Zealand white rabbits were immunized with the recombinant protein. An indirect ELISA method was used to determine serum IgG levels, through Western blotting to verify immune specificity and different stages worm protein extracts EGr_09888 protein expression. The

SUBMITTER: Pan XY 

PROVIDER: S-EPMC12678478 | biostudies-literature | 2025 Dec

REPOSITORIES: biostudies-literature