Modulating platelet-activating factor by rupatadine attenuates gentamicin-induced nephrotoxicity in rats via NF-κB/caspase-3 and Nrf2/HO-1 signaling cascades.

ABSTRACT: Gentamicin (GEN) is a commonly prescribed antibiotic for Gram-negative bacterial infections. One of the most common adverse consequences of it is renal damage which is developed in 30% of individuals receiving GEN for over 7 days. For the first time, we attempted to examine the reno-protective activity of rupatadine (RUP) on GEN-induced renal injury in rats. Renal damage was established by GEN in male Wistar rats. Histopathological analysis and kidney function panel were assessed. Levels of MDA, catalase, and SOD were detected using the colorimetric method. ELISA was utilized to assess the renal levels of IL-1β and TNF-α. qRT-PCR assessed mRNA levels of Bax and Bcl-2. Protein expression of Nrf-2, NF-κB, and caspase 3 were evaluated using Western blotting. GEN resulted in renal malfunction,

SUBMITTER: Mohyeldin RH 

PROVIDER: S-EPMC12678517 | biostudies-literature | 2025 Dec

REPOSITORIES: biostudies-literature