Hyperoside flavonoids protect against malathion-induced mitochondrial toxicity in the differentiated SH-SY5Y cells.

ABSTRACT: Malathion (MAL), an organophosphorus pesticide, is known to induce mitochondrial toxicity in neuronal cells, contributing to neurodegenerative processes. This study aims to investigate the protective effects of hyperoside (HYP), a flavonoid, against mitochondrial dysfunction induced by MAL in differentiated SH-SY5Y cells. Differentiated human neuroblastoma cell lines were treated with various concentrations of MAL (0.01 to 100 mM) and HYP (10 to 40 µM). Cell viability was assessed using MTT and BrdU assays, while mitochondrial function was evaluated through ATP production, mitochondrial membrane potential (MMP), oxygen consumption rates (OCR), mitophagy-related proteins (PARKIN and PINK1) evaluation, and expression of key mitochondrial genes (i.e., ND1/5, Cy.b, CO1, and ATP 6/8). Bioinform

SUBMITTER: Elmorsy EM 

PROVIDER: S-EPMC12678533 | biostudies-literature | 2025 Dec

REPOSITORIES: biostudies-literature