CLE19 suppresses brassinosteroid signaling output via the BSL-BIN2 module to maintain BES1 activity and pollen exine patterning in Arabidopsis.

ABSTRACT: The pollen exine serves as a protective barrier and signaling interface essential for male fertility in flowering plants. Its precise patterning depends on coordinated interactions between microspores and tapetal cells. While the CLAVATA3/EMBRYO SURROUNDING REGION-related 19 (CLE19) peptide has been identified as a microspore-derived "brake" that restricts tapetal activity to maintain exine developmental homeostasis, how CLE19 integrates with hormonal signaling pathways remains poorly understood. Here, we demonstrate that CLE19 attenuates brassinosteroid (BR) signaling output by engaging a defined BSL-BIN2-BES1 signaling cascade. Through quantitative phosphoproteomic analysis, we identified that CLE19 affects the phosphorylation of multiple BR signaling components, including BSL-type phosp

SUBMITTER: Wang S 

PROVIDER: S-EPMC12678687 | biostudies-literature | 2025 Dec

REPOSITORIES: biostudies-literature