Characterization of a Fructan Exohydrolase from the Gut Bacterium <i>Anaerostipes butyraticus</i>.

ABSTRACT: Glycoside hydrolase family 32 (GH32) enzymes play key roles in fructooligosaccharide metabolism in gut bacteria. In this study, a GH32 enzyme (GenBank code, GFO85652) containing carbohydrate binding module 66 (CBM66) from the gut bacterium Anaerostipes butyraticus (AbFEH) was heterologously expressed in Escherichia coli. We constructed an expression plasmid that does not contain sequences for the N-terminal signal peptide and the C-terminal region potentially involving cell-wall binding. The enzyme obtained (AbFEH∆C) was purified and characterized. Thin-layer chromatography and high-performance liquid chromatography analyses revealed that AbFEH∆C produced fructose from all the substrates, sucrose, 1-kestose, inulin, and levan, and intermediate oligosaccharide products were no

SUBMITTER: Baula MAB 

PROVIDER: S-EPMC12678895 | biostudies-literature | 2025

REPOSITORIES: biostudies-literature