The Impact of Methanol Concentration on Recombinant Protein Glycosylation in Pichia pastoris SuperMan5.

ABSTRACT: The methylotrophic yeast Pichia pastoris (also known as Komagataella phaffii) is a prominent platform for recombinant protein production, offering benefits such as thermo- and osmotolerance, high-density growth, and efficient protein secretion. Its ability to metabolise methanol, an increasingly available carbon source, enhances its cost-effectiveness and sustainability for industrial use. As a eukaryotic host, P. pastoris ensures proper protein folding and post-translational modifications (PTMs), including glycosylation, which is essential for correct folding and endoplasmic reticulum (ER) quality control. While ER-transferred glycans are critical for maturation, additional modification in the Golgi apparatus can yield larger glycans whose impact on stability, solubility, and bioactivity

SUBMITTER: Ohara A 

PROVIDER: S-EPMC12679317 | biostudies-literature | 2025 Dec

REPOSITORIES: biostudies-literature