Engineering and evaluation of precision-glycosylated clickable albumin nanoplatform for targeting the tumor microenvironment.

ABSTRACT: Rationale: Glycosylation of drug delivery vehicles enables selective tumor microenvironment (TME) targeting but is limited by the lack of precise glycan control and unbiased evaluation of in situ targeting. We developed a clickable albumin nanoplatform engineered by distinct glycosylation for selective in vivo cell targeting (CAN-DGIT) with a defined number of sugar moieties and integrated spatial transcriptomics (ST) to map nanoparticle-TME interactions. Methods: Albumin was functionalized with azadibenzocyclooctyne (ADIBO) at a controlled degree of functionalization (DOF), confirmed by MALDI-TOF and UV-vis spectroscopy, followed by conjugation of azide-functionalized mannose, galactose, or glucose via click chemistry. Nanoparticles were labeled with ⁶⁴Cu o

SUBMITTER: Park JY 

PROVIDER: S-EPMC12679570 | biostudies-literature | 2026

REPOSITORIES: biostudies-literature