Development of an HPLC method for the simultaneous determination of azithromycin, clarithromycin, and erythromycin in wastewater.

ABSTRACT: The widespread occurrence of macrolide antibiotics in aquatic environments poses serious ecological and public health concerns, highlighting the need for reliable, accessible, and validated analytical methods. In this study, a rapid and cost-effective high-performance liquid chromatography method with photodiode array detection (HPLC-PDA) was developed and validated for the simultaneous determination of azithromycin (AZM), clarithromycin (CLR), and erythromycin (ERY) in wastewater. Chromatographic separation was achieved on an Agilent Zorbax Eclipse C18 column (250 × 4.6 mm, 5 µm) at 35 °C using a mobile phase of acetonitrile and 10 mM NaH₂PO₄ buffer (60 : 40, v/v, pH 6.0), flow rate 0.8 mL min^-1, and injection volume 10 µL. Retention times were 3.76, 4.91,

SUBMITTER: Duong TTA 

PROVIDER: S-EPMC12679586 | biostudies-literature | 2025 Dec

REPOSITORIES: biostudies-literature