Effects of Inlet Capillary Temperature in Atmospheric-Pressure Infrared Laser-Ablation Plasma Postionization Mass Spectrometry.

ABSTRACT: Mass spectrometry imaging (MSI) can be used to survey numerous molecular species from a wide variety of surfaces, including biological tissue sections. Atmospheric-pressure (AP) infrared laser-ablation plasma postionization (IR-PPI) has recently been shown to allow matrix free analysis of small molecules from both fresh frozen and formalin fixed paraffin embedded (FFPE) tissue. Detected ion intensities in IR-PPI as well as other AP inlet modalities such as desorption electrospray ionization (DESI) show a strong dependence on the inlet capillary temperature. In this study, the relationship between detected ion intensity and inlet capillary temperature is evaluated, between room temperature and 650 °C, for analyte pipetted on various substrates, as well as fresh frozen and FFPE tissue, by IR

SUBMITTER: Ellis-Gibbings L 

PROVIDER: S-EPMC12679636 | biostudies-literature | 2025 Dec

REPOSITORIES: biostudies-literature