Photobiomodulation-pretreated dental follicle stem cells with enhanced osteogenic function improve alveolar bone regeneration.

ABSTRACT:

Background

Dental follicle stem cell (DFSC) therapy is an excellent option for reducing alveolar bone loss. Preconditioning DFSCs increases their resistance to the disease microenvironment and increases their efficacy. Photobiomodulation therapy (PBMT) is an effective and convenient way to precondition DFSCs. In this study, we evaluated the effects of PBMT-pretreated DFSCs on alveolar bone regeneration and investigated the underlying mechanism.

Methods

After DFSCs were isolated and characterized, various biological properties of DFSCs following treatment with PBMT were evaluated in viability tests, wound healing assays, alizarin red staining, alkaline phosphatase staining, real-time quantitative PCR, mitochondrial membrane potential assays, and ATP and reactive oxygen specie

SUBMITTER: Li A 

PROVIDER: S-EPMC12679786 | biostudies-literature | 2025 Dec

REPOSITORIES: biostudies-literature