Ferroptosis-mediated anticancer activity of endoperoxide-containing steroids derived from <i>Daedaleopsis confragosa</i> via targeting NOS2.

ABSTRACT: Mushrooms belonging to the genus Daedaleopsis are known to contain various biologically active constituents, including steroids. However, they have not been studied as extensively as other medicinal mushrooms. In this study, chemical analysis was performed on the methanolic extract of the fruiting bodies of D. confragosa, a species that has not been thoroughly explored. Seven steroidal compounds were identified, and among these compounds, 1, 2, 3 and 5, which contain an endoperoxide bridge, exhibited strong cytotoxicity against HCT116, A549, MDA-MB-231, and A375SM cancer cell lines. Computational target prediction identified inducible nitric oxide synthase (NOS2) as their most probable molecular target. Based on this, compound 2, with the mo

SUBMITTER: Heo EJ 

PROVIDER: S-EPMC12679854 | biostudies-literature | 2026

REPOSITORIES: biostudies-literature