Genome resequencing and custom genotyping elucidates the origin and dissemination history of an emblematic grapevine cultivar, 'Tempranillo Tinto'.

ABSTRACT: Grapevine cultivars are vegetatively propagated to maintain their varietal characteristics. However, long periods of cultivar multiplication result in the accumulation of spontaneous somatic mutations that can differ among clonal lines. Here, we explored this intravarietal genetic diversity to trace back the origin and dissemination history of 'Tempranillo Tinto', the third most cultivated wine grape variety worldwide. A stringent somatic variant calling over whole-genome resequencing data of 35 'Tempranillo Tinto' grapevines from seven Iberian winemaking regions revealed 158 somatic single nucleotide variants (SNVs) shared by some of the plants. Among them, 56 highly informative SNVs were used to custom-design a high-throughput intravarietal genotyping assay, which was validated and used

SUBMITTER: Tello J 

PROVIDER: S-EPMC12679915 | biostudies-literature | 2025 Dec

REPOSITORIES: biostudies-literature