Development and evaluation of loop-mediated isothermal amplification (lamp) for rapid detection of campylobacter jejuni.

ABSTRACT:

Objectives.

To develop and evaluate a loop-mediated isothermal amplification (LAMP) assay for the rapid detection of Campylobacter jejuni in stool samples associated with acute diarrheal disease (ADD) and Guillain-Barré syndrome (GBS) for implementation in regional laboratories and primary health care centers.

Materials and methods.

Four sets of LAMP primers for C. jejuni were designed and initially evaluated with DNA from C. jejuni, Campylobacter coli, Salmonella Infantis, and Escherichia coli extracted using a commercial kit. The best-performing primer set was selected for assay optimization, determination of the limit of detection, and analytical validation with DNA from the aforementioned strains. Subsequently, clinical validation was performed using stool samples from p

SUBMITTER: Caro-Castro J 

PROVIDER: S-EPMC12679974 | biostudies-literature | 2025 Dec

REPOSITORIES: biostudies-literature