Integrating single-cell biophysical and transcriptomic features to resolve functional heterogeneity in mantle cell lymphoma.

ABSTRACT: Intratumor heterogeneity impacts disease progression and therapeutic resistance but remains poorly characterized by conventional histologic, immunophenotypic, and molecular approaches. Single-cell biophysical properties distinguish functional phenotypes complementary to these approaches, providing additional insight into cellular diversity. Here, we link both buoyant mass and stiffness to gene expression to identify clinically relevant phenotypes within primary mantle cell lymphoma (MCL) cells, using MCL as a model of biological and clinical diversity in human cancer. Linked measurements reveal that buoyant mass and stiffness characterize B cell development states from naïve to plasma cell and correlate with expression of oncogenic B cell receptor signaling genes such as BLK and

SUBMITTER: Zhang Y 

PROVIDER: S-EPMC12680032 | biostudies-literature | 2025 Dec

REPOSITORIES: biostudies-literature