Conservation of mRNA operon formation in control of the heat shock response in mammalian cells.

ABSTRACT: Prokaryotes use polycistronic transcription (operons) to express multiple messenger RNAs (mRNAs) from a single promoter to coexpress functionally related genes. However, how do eukaryotes, which express monocistronic messages, achieve the same regulation? Previously, we demonstrated that yeast uses RNA operons, i.e., mRNAs assembled in trans (transperons), to control multiple cellular pathways such as the heat shock response (HSR). As the HSR is conserved from yeast to mammals, we used single-molecule RNA labeling and pulldown techniques to demonstrate that mammalian heat shock protein (HSP) mRNAs also form operons upon transcription during heat stress. HSP RNA operon formation is dependent on the heat shock factor 1 transcription factor and intra- and interchromosomal interactions between

SUBMITTER: Pataki E 

PROVIDER: S-EPMC12680049 | biostudies-literature | 2025 Dec

REPOSITORIES: biostudies-literature