Kinetics of aldosterone-dependent ENaC trafficking in the kidney.

ABSTRACT: The epithelial Na channel (ENaC) is a heterotrimer whose trafficking to the apical membrane is stimulated by aldosterone. Trafficking is associated with proteolytic cleavage of the α and γ subunits. We examined the kinetics of this process to ascertain whether the observed changes could contribute to the most rapid anti-natriuretic effects (within 1-3 h) of hormone administration in rats. Infusion of aldosterone increased the abundance of cleaved αENaC and γENaC with time constants of 2.2 and 2.3 h, respectively. Decreases in full-length γENaC and increases in full-length αENaC occurred more slowly, with time constants of 22 and 17 h. Decreases in aldosterone also caused rapid decreases in cleaved and slower changes in full-length forms. Kinetic modeling suggested that the major effect of

SUBMITTER: Frindt G 

PROVIDER: S-EPMC12680067 | biostudies-literature | 2025 Sep

REPOSITORIES: biostudies-literature