Universal Nanopore Sensor for Amplification-Free and Label-Free Detection of Molecular Biomarkers.

ABSTRACT: Molecular diagnostics of biomarkers derived from tissues and bodily fluids are developing into a cornerstone of medical testing and potentially the foundation of a personalized medicine approach. Therefore, there is a need for universal biosensors capable of detecting different biomarker types with high performance and minimal complexity, ideally suitable for point-of-care use. Here, we present an integrated nanopore sensor for amplification-free, label-free, and quantitative detection of both proteins and nucleic acids from clinical samples. High performance is enabled by trapping target-carrying microbeads at the nanopore location and rapid detection of individual targets upon release from the beads. Low complexity is achieved via a simple solid-phase extraction protocol and optics-free,

SUBMITTER: Xu R 

PROVIDER: S-EPMC12680103 | biostudies-literature | 2025 Aug

REPOSITORIES: biostudies-literature