Single sperm karyotyping of testicular sperm in non-obstructive and obstructive azoospermia using next generation sequencing.

ABSTRACT: The sperm of infertile men have higher rates of chromosomal abnormalities than those of fertile men. Miscarriage rate is also higher following testicular sperm extraction combined with intracytoplasmic sperm injection (TESE-ICSI). Sperm chromosomal abnormalities are assumed to be the cause of miscarriages. Previous testicular sperm karyotyping studies have only examined a few selected chromosomes using fluorescence in situ hybridization. The aim of this study was to provide a more detailed analysis of sperm karyotyping by analyzing all chromosomes using next-generation sequencing (NGS) in clinically usable testicular sperm. Sperm discarded after clinical use was collected for NGS. Additionally, sperm were individually collected by micromanipulation from patients with obstructive azoospermi

SUBMITTER: Sueyoshi S 

PROVIDER: S-EPMC12680224 | biostudies-literature | 2025

REPOSITORIES: biostudies-literature