Development and validation of a quantitative real-time PCR assay for the enumeration of Clostridium sporogenes in NaCl- and nitrite-reduced meat products.

ABSTRACT: In the interest of enhancing consumer health and limiting the risks associated with excessive salt consumption and nitrosamine intake, there is an ongoing initiative to reduce the use of salt and nitrite in meat products. Since important microbial barriers are compromised in their capacity to function effectively, NaCl- and nitrite-reduced products also run the risk of increasing the growth of potentially pathogenic microorganisms, such as Clostridium botulinum. In consideration of the necessity to investigate the growth behaviour of the pathogen in sodium chloride (NaCl) - and nitrite-reduced meat products in future challenge tests, a real-time PCR assay was developed and validated for the qualitative and quantitative detection of the C. botulinum surrogate Clostridium sporogenes. The qua

SUBMITTER: Schuller N 

PROVIDER: S-EPMC12680228 | biostudies-literature | 2025

REPOSITORIES: biostudies-literature