Hiding in plain sight: Genomic and phenotypic characterization of mosquito-borne Bussuquara virus.

ABSTRACT: Bussuquara virus (BSQV), an orthoflavivirus discovered in Brazil in 1956, has been detected throughout the Americas in diverse mosquito and vertebrate species, including humans. Critical gaps in BSQV knowledge include its capacity for urban transmission and clinical pathogenesis outcomes, with insufficient historical experimentation to draw genomic or phenotypic comparisons to related orthoflavivirus species. The objective of this study was to conduct morphologic, genomic, phylogenetic, and in vitro viral fitness characterization of BSQV using the four available historical strains. We used next generation sequencing and rapid amplification of cDNA ends to construct consensus genomes, followed by phylogenetic analysis and genome annotation to evaluate orthoflavivirus evolutionary relationsh

SUBMITTER: Steck MR 

PROVIDER: S-EPMC12680234 | biostudies-literature | 2025 Dec

REPOSITORIES: biostudies-literature