Targeting and anchoring the mechanosensitive ion channel Piezo to facilitate its inhibition of axon regeneration.

ABSTRACT: Mechanical force orchestrates a myriad of cellular events including inhibition of axon regeneration, by locally activating the mechanosensitive ion channel Piezo enriched at the injured axon tip. However, the cellular mechanics underlying Piezo localization and function remains poorly characterized. We show that the RNA repair/splicing enzyme Rtca acts upstream of Piezo to modulate its expression and transport/targeting to the periphery of the soma via the Rab10 GTPase, whose expression also relies on Rtca. Loss or gain of function of Rab10 promotes or impedes Drosophila sensory neuron axon regeneration, respectively. Rab10 mediates the cell surface expression of integrin β1 (Itgb1)/mys, which colocalizes and genetically interacts with Piezo, facilitating its anchorage and engagement with

SUBMITTER: Wang Q 

PROVIDER: S-EPMC12680355 | biostudies-literature | 2025 Dec

REPOSITORIES: biostudies-literature