Protein inhibitor of activated STAT 2 (PIAS2) protects against podocyte injury in diabetic nephropathy via inhibiting JAK2/STAT3 pathway.

ABSTRACT:

Background

Diabetic nephropathy (DN) is a common complication in diabetic patients, and the pathological mechanism has not been fully understood. PIAS2, as a counter regulator of the JAK2/STAT3 signaling pathway, may play a crucial role in DN. This study aims to investigate the expression of PIAS2 in high glucose-induced podocytes and the protective effect against podocyte cell injury.

Methods

Human kidney podocytes were cultured under normal glucose (5.5 mM) and high glucose (30 mM) conditions. The expression of PIAS2 was detected by RT-qPCR and western blotting. Subsequently, the effects of PIAS2 overexpression in high glucose induced human podocytes were evaluated through the determination of cell viability, apoptosis, and secretion of inflammatory cytokines. Recombinant

SUBMITTER: Li X 

PROVIDER: S-EPMC12680398 | biostudies-literature | 2025 Nov

REPOSITORIES: biostudies-literature