Multi-Omics Analysis and Experimental Validation Identify RAD51 as a Key Biomarker in OSCC.

ABSTRACT: Oral squamous cell carcinoma (OSCC) is an aggressive malignancy associated with high morbidity and mortality. RAD51 recombinase (RAD51), a central DNA repair protein, plays a crucial role in homologous recombination and has been implicated in cancer progression through mechanisms such as genomic instability, chemoresistance and immune modulation. However, its specific function and regulatory mechanisms in OSCC remain incompletely elucidated. We conducted an integrated multiomics analysis including differential expression, single-cell transcriptomics, prognostic evaluation, functional enrichment and immune infiltration profiling. Experimental validation was performed using siRNA-mediated RAD51 knockdown in OSCC cell line HSC-3, followed by functional assays to assess proliferation, migratio

SUBMITTER: Shi Y 

PROVIDER: S-EPMC12680491 | biostudies-literature | 2025 Jan-Dec

REPOSITORIES: biostudies-literature