Targeting CPS1 attenuates lung cancer metastasis by regulating EMT through an epigenetic mechanism.

ABSTRACT: Background: Metastasis is the primary cause of cancer-related mortality, and targeting the drivers of this process is a promising strategy to improve patient outcomes. Recent studies have highlighted a role of Carbamoyl Phosphate Synthetase 1 (CPS1), the urea cycle's rate-limiting enzyme, in tumor development. However, its involvement in tumor spreading and metastasis remains unclear. Methods: Transwell assay, wound healing assay and a range of lung cancer metastasis animal models were employed to investigate the impact of genetic knockdown and pharmacological inhibition of CPS1 on lung cancer metastasis both in vitro and in vivo. Quantitative proteomic analysis, RNA sequencing, untargeted metabolomics and targeted metabolomics to urea cycle were conducted to el

SUBMITTER: Ding Y 

PROVIDER: S-EPMC12680530 | biostudies-literature | 2026

REPOSITORIES: biostudies-literature