Single cell and spatial transcriptomic profiling of the type 2 diabetic coronary microcirculation and myocardium.

ABSTRACT: Coronary microvascular disease (CMD) is an early complication of type 2 diabetes (T2D) involving adverse endothelial and smooth muscle function, vascular remodeling, and alterations in mechanics. These culminate in impaired coronary blood flow. To interrogate transcriptional differences potentially contributing to CMD, we tested the hypothesis that comprehensive single-cell and spatial transcriptomic profiling of the coronary microcirculation and surrounding myocardium will identify new pathways to target in CMD. We utilized an innovative combination of single-cell RNA profiling and spatial transcriptomics to examine transcriptional differences and molecular signatures of CMD in T2D mice. Single-cell RNA profiling and spatial transcriptomics revealed an upregulation of genes linked to adip

SUBMITTER: McCallinhart PE 

PROVIDER: S-EPMC12680737 | biostudies-literature | 2025 Dec

REPOSITORIES: biostudies-literature