Comparative evaluation of comprehensive offline 2D-LC strategies coupled to MS for untargeted metabolomic studies of human urine.

ABSTRACT: Out of the broad selection of analytical methods applied in metabolomic studies, liquid chromatography coupled to mass spectrometry (LC-MS) has the highest coverage potential. In that regard, the quality of the separation process is crucial for the analytical outcome. Reversed-phase (RP) and hydrophilic interaction liquid chromatography (HILIC) are widely applied, and the potential of various setups to combine these modes for more complementary data has been deeply explored. In our previous study on the orthogonality of LC conditions in the field of metabolomics, combinations of a mixed-mode phase with parallel RP and ion-exchange (IEX) properties and several HILIC columns exhibited the widest compound distributions in a two-dimensional (2D) separation space. For further performance evalua

SUBMITTER: Grubner M 

PROVIDER: S-EPMC12680765 | biostudies-literature | 2025 Dec

REPOSITORIES: biostudies-literature