LegioTyper: Rapid typing of Legionella pneumophila serogroup 1 by flow-based chemiluminescence sandwich microarray immunoassay.

ABSTRACT: Chemiluminescence imaging of a sandwich microarray immunoassay (CL-SMIA) is a simple and rapid method for antibody-based serotyping of pathogens like L. pneumophila, which is important to identify even outbreak-associated subtypes like L. pneumophila Sg 1 subtype Philadelphia. The CL image pattern can be interpreted by a decision path similar to the well-known "Dresden Panel", which is typically performed via indirect ELISA. New antibodies needed to be characterized for the so-called LegioTyper panel as capturing antibodies to perform the flow-based CL-SMIA. The following was achieved for the LegioTyper principle: (i) minimizing the costs for antibody microarrays by manufacturing Jeffamine-2003-coated polycarbonate sheets, (ii) optimized automated test procedure which is able to perform th

SUBMITTER: Barwinkel C 

PROVIDER: S-EPMC12680792 | biostudies-literature | 2025 Dec

REPOSITORIES: biostudies-literature