Signal enhancement of lipids and glycans using ammonium fluoride for IR-MALDESI mass spectrometry imaging.

ABSTRACT: Mass spectrometry imaging (MSI) is an analytical technique that enables the simultaneous detection and localization of hundreds to thousands of molecules across a tissue surface. The addition of ammonium fluoride to the electrospray or to tissue samples has been shown to increase the signal of lipids in both nano-DESI-MSI and MALDI-MSI, respectively. Herein, this strategy is applied to infrared matrix-assisted laser desorption electrospray ionization (IR-MALDESI) which demonstrated signal enhancement for both lipids and N-linked glycans using 70 µM and 350 µM NH₄F in 50% ACN with 1 mM acetic acid as the ESI solvent, respectively. Up to a 93-fold increase in signal was observed for these biomolecules without any change in sample preparation or to the mass spectrometer parameters,

SUBMITTER: Eisenberg SM 

PROVIDER: S-EPMC12680844 | biostudies-literature | 2025 Dec

REPOSITORIES: biostudies-literature