C-type natriuretic peptide mitigates apoptosis in ovarian granulosa cells through the cGMP pathway independent of PKG signaling.

ABSTRACT:

Background

Mammalian follicular development is regulated by endocrine and paracrine factors, and apoptosis of granulosa cells represents a major cause of follicular atresia. C-type natriuretic peptide (CNP), a paracrine factor in follicular fluid, maintains meiotic arrest of oocytes through activation of the NPR2-cGMP pathway. Protein kinase G (PKG) is a principal downstream effector of cGMP, but its function in granulosa cell apoptosis has not been fully characterized.

Methods

Bovine granulosa cells were treated with CNP, cGMP or the PKG inhibitor KT5823. Transcriptomic analysis was performed to identify differentially expressed genes and signaling pathways. The effects of CNP on apoptosis were further evaluated in mural granulosa cells, cumulus-oocyte complexes (COCs), and

SUBMITTER: Wei Y 

PROVIDER: S-EPMC12681114 | biostudies-literature | 2025 Dec

REPOSITORIES: biostudies-literature