Pinoresinol enhances intestinal tight junction integrity via activation of the CaMKKβ-AMPKα1 pathway in Caco-2 cells.

ABSTRACT:

Background

The intestinal epithelial barrier plays an essential role in protecting the body while allowing selective nutrient absorption. Natural bioactive compounds that strengthen this barrier contribute to gut health. However, the effects of pinoresinol, a dietary lignan known for its various bioactivities, on intestinal barrier function and underlying molecular mechanisms remain to be elucidated.

Methods

Caco-2 monolayers were treated with pinoresinol, and barrier function was evaluated using fluorescein permeability and transepithelial electrical resistance (TEER) assays. The expression and localization of tight junction (TJ)-related proteins were analyzed by RT-PCR, western blotting, and immunofluorescence. The involvement of the CaMKKβ-AMPKα1 signaling pathway was als

SUBMITTER: Shin SB 

PROVIDER: S-EPMC12681162 | biostudies-literature | 2025 Dec

REPOSITORIES: biostudies-literature