Impact of fiber molecular structure on resistance to digestion using the infogest and rat small intestine extract protocols.

ABSTRACT:

Purpose

Dietary fibers differ in their molecular structure, which influences their breakdown under digestive conditions. This study investigates how fiber molecular structure affects resistance to digestion using in vitro models.

Methods

High-, medium-, and low-molecular-weight (Mw) corn arabinoxylans, resistant maltodextrin, and inulin were characterized by carbohydrate composition following acid hydrolysis and analyzed for sugar release during simulated digestion using the standardized InfoGest protocol and rat small intestinal extract (RSIE).

Results

High-Mw corn arabinoxylan (208.8 kDa) remained largely stable, with minor, non-significant increases in glucose and galactose. Medium- (25.5 kDa) and low-Mw (2.5 kDa) arabinoxylans showed partial hydrolysis, with incre

SUBMITTER: Boukid F 

PROVIDER: S-EPMC12681484 | biostudies-literature | 2025 Dec

REPOSITORIES: biostudies-literature