Novel multitarget ACE inhibitory peptides from grass carp swim bladder: Identification and potential antihypertensive mechanism.

ABSTRACT: Sustainable utilization of aquaculture byproducts is imperative for advancing circular bioeconomy in food systems. This study developed an integrated in silico-in vitro screening strategy to identify novel angiotensin-converting enzyme (ACE) inhibitory peptides from grass carp swim bladder hydrolysates. Two novel peptides, FPSPFR (749.86 Da) and LWDPPEGAP (981.06 Da), were screened with IC₅₀ values of 81.34 μM and 206.91 μM, respectively. Kinetic characterization revealed non-competitive inhibition alongside exceptional gastrointestinal stability. Molecular docking showed strong binding affinities (-10.0 and -9.8 kcal/mol) via hydrogen bonding and hydrophobic interactions, while molecular dynamics simulations confirmed stable binding conformations. Network p

SUBMITTER: Dong Y 

PROVIDER: S-EPMC12681514 | biostudies-literature | 2025 Dec

REPOSITORIES: biostudies-literature