ExoOrb: A novel visual and analytical system for therapeutic extracellular vesicles metrics.

ABSTRACT: Extracellular vesicles (EVs) are naturally secreted nanoscale mediators of intercellular communication, showing potential for therapeutic and functional food applications. Although many EVs are being isolated with claims of therapeutic benefits, the evaluation criteria require extensive resources and time, often resulting in futile outcomes. This work addresses this gap by developing a visual and quantitative system using monk fruit cell-derived EVs (MFEVs) as a model to efficiently select the most suitable therapeutic EVs by analyzing their characterization parameters. This approach saves valuable resources and time. To generate variations, MFEVs were isolated using eight different techniques: ultracentrifugation, ultrafiltration, polyethylene glycol (PEG) precipitation (8 %, 10 %, 15 %,

SUBMITTER: Rehman TU 

PROVIDER: S-EPMC12681852 | biostudies-literature | 2025

REPOSITORIES: biostudies-literature