NEAT1 is a therapeutic target for reversing T-cell exhaustion in bladder cancer.

ABSTRACT:

Background

T-cell exhaustion induced by the tumor microenvironment is an important factor in posing a major challenge to effective cancer immunotherapy. Immune checkpoint inhibitors aim to reverse T-cell exhaustion. However, the effectiveness of immune checkpoint inhibitors is often limited due to their off-target effects and single targets. Herein, we attempt to identify molecular targets that can regulate the expression of multiple immune checkpoints to reverse T-cell exhaustion.

Methods

NSG mice with xenotransplantation of human bladder cancer cells were used to investigate the function of nuclear paraspeckle assembly transcript 1 (NEAT1) in T-cell exhaustion. Chromatin isolation by RNA purification, chromatin immunoprecipitation, and luciferase assays was employed to inv

SUBMITTER: Li K 

PROVIDER: S-EPMC12682204 | biostudies-literature | 2025 Dec

REPOSITORIES: biostudies-literature