Regulated Release of Small Extracellular Vesicles Directs Neutrophil Recruitment in Cutaneous Wound Healing.

ABSTRACT: Normal cutaneous wound healing is a multicellular process that involves the release of small extracellular vesicles (sEVs) that coordinate intercellular communication by delivery of sEV payloads to recipient cells. We have recently shown how the proreparative activity of inflammatory cell sEVs, especially macrophage and neutrophil-derived sEVs, in the wound bed is dysregulated in impaired wound healing. In this study, we show that loss of Rab27A, a small GTPase that has a regulatory function in sEV secretion, reduces the release of neutrophil and macrophage-derived sEVs. Proteomic profiling of sEVs identified Rab27A-dependent protein payloads relevant in neutrophil activation, such as CD44, leukotriene A4 hydrolase, complement C5, and neutrophilic granule protein. We defined the activity o

SUBMITTER: Choi W 

PROVIDER: S-EPMC12682223 | biostudies-literature | 2026 Mar

REPOSITORIES: biostudies-literature