Engineering Cortical Networks: An Open Platform for Controlled Human Circuit Formation and Synaptic Analysis In Vitro.

ABSTRACT: Neuronal circuits are organized by specific connections between neuron types across various brain regions. Understanding how these circuits form is crucial for uncovering the mechanisms behind circuit-related dysfunction in brain diseases. Human-induced pluripotent stem cell (iPSC) models enable the study of the molecular and cellular processes underlying neuronal networks, but their lack of precise architecture limits the investigation of specific neuronal interactions and activity-dependent processes. Microfluidic technologies offer structural control but are confined by closed systems that restrict 3D network integration, scalability, and cell retrieval. To overcome these challenges, we developed an open cortical network platform that integrates iPSC-derived cortical neurons with bioeng

SUBMITTER: Suklai P 

PROVIDER: S-EPMC12683197 | biostudies-literature | 2025 Dec

REPOSITORIES: biostudies-literature