Coordinated Development of Immune Cell Populations in Vascularized Skin Organoids from Human Induced Pluripotent Stem Cells.

ABSTRACT: Human skin is a highly vascularized organ, where blood vessels perform essential roles in maintaining skin homeostasis and participate in thermal regulation. Currently, there is an unmet challenge to generate vascularized, functional skin models. In this study, human fetal placental endothelial colony forming cells (ECFC) are incorporated into human induced pluripotent stem cell (hiPSC)-derived skin organoids (SKO), forming capillary-like structures and generating endothelialized SKOs. However, this approach is limited by the inability of ECFCs to establish a complete vascular network, and impeding full epidermal stratification and hair follicle morphogenesis. In independent experiments, hiPSC-derived vascular organoids (VO), are incorporated into the SKO, forming complex vascular structur

SUBMITTER: Mostina M 

PROVIDER: S-EPMC12683213 | biostudies-literature | 2025 Dec

REPOSITORIES: biostudies-literature