Safety profile of lutein-based blue dyes and surgical lights.

ABSTRACT:

Introduction

The combination of surgical endoillumination and vital dyes has been associated with phototoxicity and retinal damage, despite vital dyes being mainly considered safe. Based on this perspective, the aim of the present study was to evaluate the effect of lutein-based blue dyes (LBBDs) on human retinal pigmented epithelial (ARPE-19) cells and their combination with surgical light exposure.

Methods

ARPE-19 cells were exposed to LBBDs and/or xenon/LED light according to the following experimental design: phase I, exposure to LBBDs only; phase II, exposure to lights only; phase III, single sequential exposure to LBBDs and xenon/LED light; and phase IV, double sequential exposure to LBBDs and xenon/LED light. ATPlite and reactive oxygen species (ROS) detection assays

SUBMITTER: Romano MR 

PROVIDER: S-EPMC12683236 | biostudies-literature | 2025

REPOSITORIES: biostudies-literature