Viability of Self-Taken Vaginal Swab Samples for RNA-Based Biomarker Analysis in Cervical Disease.

ABSTRACT: High risk human papillomaviruses (hrHPVs) cause most cervical cancers. Cervical screening programmes aim to identify precancerous disease using detection of hrHPV nucleic acid using clinician-taken liquid-based cytology (LBC) samples, or increasingly, self-taken samples (STSs). However, STSs are incompatible with traditional cytology triage. Therefore, development of novel triage tests is essential. Quantification of cellular and viral mRNA biomarkers is one option, but little is known about mRNA quality in STSs. We extracted RNA from two sets of STSs (reflecting separate sampling devices) from the Scottish HPV Archive (SHA) and the Swedish Cervical Cytology Biobank (SCCB). We investigated whether the ACTB, GAPDH and p16 RNAs could be amplified by reverse transcription quantitative PCR (RT

SUBMITTER: Scott H 

PROVIDER: S-EPMC12683698 | biostudies-literature | 2025 Dec

REPOSITORIES: biostudies-literature