Establishment and Characterization of Bovine Trophoblast Stem Cells Growing as Adherent Cells or Vesicles.

ABSTRACT: In ungulates, the development of the trophoblast lineage follows a peculiar process, as implantation is preceded by a relatively long period of trophoblast elongation, the molecular control of which is still not well known. Here, we have established novel bovine trophoblast stem cells (b2iTSCs) growing in a completely defined medium containing inhibitors of GSK3b and MEK, and WNT3 during establishment. These cells can grow either as adherent, or as vesicles in suspension. These fluid-filled vesicles show high proliferative capacity and apical polarity. Transcriptome analysis reveals that b2iTSCs are distinct from bovine embryonic fibroblasts and exhibit gene expression profiles related to lipid metabolism, steroid hormone synthesis, and nutrient absorption. The cells also express different

SUBMITTER: Baquerre C 

PROVIDER: S-EPMC12683708 | biostudies-literature | 2025 Dec

REPOSITORIES: biostudies-literature