Research of antimicrobial resistance and its associated genes distribution in <i>Escherichia coli</i> from diarrheic calves in the Ulagai region of China.

ABSTRACT: As a conditional pathogenic bacterium, Escherichia coli is a major contributor to infect calf diarrhea. It has attracted extensive attention due to antimicrobial resistance (AMR) and pathogenicity. To elucidate the AMR profiles and resistance-related genes in E. coli isolated from calf diarrhea samples in the Ulagai region E. coli was isolated and identified from samples of calf feces using E. coli chromogenic medium, Gram staining, and 16S rRNA sequencing. The antimicrobial susceptibility was tested using the Kirby-Bauer disk diffusion method. Resistance genes were analyzed using PCR. Additionally, strains showing severe multidrug resistance were selected for whole-genome sequencing. Multidrug resistance was observed in all 50 isolated E. coli strains. T

SUBMITTER: Gao W 

PROVIDER: S-EPMC12683719 | biostudies-literature | 2025

REPOSITORIES: biostudies-literature