Cleared tissue dual-view oblique plane microscopy.

ABSTRACT: We present a dual-view oblique plane microscope (dOPM) for imaging thick optically cleared tissue samples using a silicone immersion primary objective. The custom-designed remote refocusing relay utilizes stock optics to achieve remote refocusing in refractive index-matched samples. The spatial resolution of the system was characterized using a series of fluorescent bead phantoms with refractive indices ranging from 1.4-1.5, with the point spread function full width at half maximum measuring ∼0.5 µm laterally and ∼1 µm axially for a refractive index-matched bead sample, with minimal degradation over a > 250 µm axial range. We characterize how the remote refocusing performance at sample refractive indices up to n = 1.5 can be partially compensated for using adjustment of the correcti

SUBMITTER: Dvinskikh L 

PROVIDER: S-EPMC12684026 | biostudies-literature | 2025 Sep

REPOSITORIES: biostudies-literature