Quantification of oxidized and reduced cytochrome-c-oxidase by combining discrete-wavelength time-resolved and broadband continuous-wave near-infrared spectroscopy.

ABSTRACT: Quantification of cytochrome-c-oxidase (CCO) can directly inform about cerebral metabolic capacity and function, but limited options currently exist for its in vivo assessment. Near-infrared spectroscopy (NIRS) has the potential to quantify CCO and its redox states, but hyperspectral absorption measurements are required due to their broad absorption profiles and low concentrations relative to hemoglobin. While this may be achieved with continuous-wave broadband NIRS (bNIRS), separating the signal contributions of absorption and scattering remains a challenge. Alternatively, time-resolved NIRS (trNIRS) can directly disentangle absorption and scattering but is typically constrained to a few wavelengths. This work aimed to develop an approach for quantifying absolute CCO concentration using d

SUBMITTER: Eskandari R 

PROVIDER: S-EPMC12684037 | biostudies-literature | 2025 Sep

REPOSITORIES: biostudies-literature