Feasibility of diffuse Raman spectroscopy to detect in-vivo molecular changes in the tissue induced by subcutaneous implants.

ABSTRACT: Biomaterials are being designed for a broad range of medical applications, but regardless of their endpoint use, they trigger host immune responses, also known as foreign body responses (FBR). This study investigates the feasibility of using diffuse Raman spectroscopy (DRS) to detect in-vivo molecular changes in subcutaneous tissue situated immediately below the dermis. A mouse model of FBR after subcutaneous implantation of woven polyethylene terephthalate (PET) fibres is used to investigate this approach. Numerical modelling of light propagation in tissue is used to optimise the DRS instrument to detect Raman signals related to FBR-induced collagen deposition in tissue surrounding the PET fibres. Post-mortem in-situ measurements on mice (day 14, 21 and 28 post-implantation)

SUBMITTER: Dooley M 

PROVIDER: S-EPMC12684047 | biostudies-literature | 2025 Sep

REPOSITORIES: biostudies-literature