A Photoswitchable HaloTag for Spatiotemporal Control of Fluorescence in Living Cells.

ABSTRACT: Photosensitive fluorophores, whose emission can be controlled using light, are essential for advanced biological imaging, enabling precise spatiotemporal tracking of molecular features and facilitating super-resolution microscopy techniques. Although irreversibly photoactivatable fluorophores are well established, reversible reporters that can be reactivated multiple times remain scarce, and only a few have been applied in living cells using generalizable protein labeling methods. To address these limitations, we introduce chemigenetic photoswitchable fluorophores, leveraging the self-labeling HaloTag protein with fluorogenic rhodamine dye ligands. By incorporating a light-responsive protein domain into HaloTag, we engineer a tunable, photoswitchable HaloTag (psHaloTag), which can reversib

SUBMITTER: Walterspiel F 

PROVIDER: S-EPMC12684303 | biostudies-literature | 2025 Dec

REPOSITORIES: biostudies-literature